InquiryCopy Number Variation (CNV) is one of the major pathogenic genetic factors in humans. Studies have shown that CNV plays an important role in the development of diseases, especially psychiatric disorders. In traditional CNV detection strategies, the CNV of cell subtypes within one sample may be masked by a population average. In contrast, the single-cell CNV technology focuses on detecting the CNV of one single cell, reflecting information at the individual level, providing a comprehensive solution for studying disease pathogenesis, revealing genomic heterogeneity, and understanding the clonal evolution process at the single-cell level.
CD Genomics' Single-cell CNV Detection service applies the 10x Genomics Chromium technology platform to capture thousands of cells simultaneously and detect CNV at the single-cell level. This technique detects as low as 1% of rare clones and reserves broad application prospects in tumor heterogeneity and tissue typing. We provide a comprehensive and scalable solution for uncovering genomic heterogeneity and understanding clonal evolution, studying disease pathogenesis, and characterizing neuronal mosaicism at the single-cell level.
Features of the Single-cell CNV Solution:
- Accurate analysis: precise detection of single-cell CNV with a resolution of 2 Mb
- Hundreds to thousands of cell maps can be obtained
- Comprehensive analysis: hierarchical clustering analysis of single-cell CNV profiles
- Up to 100 Kb CNV can be detected within a cell population
- Wide detection range: detection of rare clonal types as low as 1 in 1,000 cells
- Wide range of applications: cell lines, primary cells, fresh and frozen tissue samples
- Analysis major: powerful analysis software for results visualization
Workflow of Single-cell CNV Solution
In contrast to the one-step capture method of the 10x Genomics single-cell transcriptome, the single-cell CNV assay requires two capture steps. During the first step, individual cells are captured using a microfluidic chip to form cell beads (CBs) containing the cells. Then CBs are treated to lyse the cells and remove all nucleus proteins, thus maintaining genomic DNA trapped in the CBs. During the later capturing step, CBs are combined with gel beads (GBs) containing the barcodes to form the labeled single-cell fractions, Cell Beads Barcoded Gel Beads (CBGBs) in microfluidics. As a result, single-cell genomic DNA is captured in two steps and tagged with barcodes, and these barcodes are used in subsequent sequencing analysis as the only evidence of cell origin.
Bioinformatics Analysis Process
Research Field:
- Oncology
- Neuroscience
- Genetics
- Stem cell biology
- Epidemiology
Applications:
- Tumor heterogeneity
- Cell line QC/certification
- Neuronal chimeras
- Understanding clonal evolution
- Genetic diseases, metabolic disorders
