InquiryThe nuclear organization represents the dynamic three-dimensional structure of the genome and its various regulatory components and is thought to play an important role in regulating genomic activity. And the structure of chromosomal regions has also been studied and proven to be related to transcriptional activity. Determining the relationship between chromosome structure, nucleosomes, chromatin state, and gene expression is one of the important goals in nuclear organization studies.
Figure 1. Chromosome territory organization in the nucleus. (Lever E., et al., 2010)
Our nuclear organization analysis service at the level of spatial discrimination
The main methods for examining nuclear organization are sequencing-based genomic methods and microscopic methods. CD Genomics combines genomic analysis with transcripts as well as histone modifications and subnuclear structures to form a complete integrated tool to image chromosomes as well as precisely aligned nucleosomal and chromatin markers in the same cell.
Figure 2. Detailed schematics of the integrated spatial genomics approach with DNA seqFISH+, RNA and intron seqFISH and multiplexed. (Takei Y., et al., 2021)
We use a multiplexed spatial multimodal approach to analyze chromosome structure, nucleosomes, chromatin states, and gene expression within the same individual cell. For the heterogeneity that exists in chromosome structure in a single cell, we are also able to precisely align images in multiple modes, allowing researchers to observe invariant features in the nucleus. This allows us to provide a spatial multi-omics dimensional solution allowing researchers to explore nuclear organization-related questions in many biological contexts.
Our advantages
- Allows direct imaging of chromosomes and nucleosomes.
- Very powerful in mapping genome-wide chromosomal interactions and has been scaled down to the single-cell level.
- Ability to analyze chromosome structure, nucleosomes, chromatin state, and gene expression within the same single cell.
- Ability to observe the heterogeneity of chromosome structure in a single cell.
You may need our services in a variety of fields and studies
- Understanding how genetic alterations occur in cancer cells.
- Differentiate between cancerous and benign tissues with high precision.
- Assess the function of abnormally expressed nuclear matrix proteins in diseases such as cancer.
- Discover new disease markers that can be used as a basis for improving diagnosis, predicting prognosis, and designing and monitoring new treatments.
References
- Lever E., et al., (2010). "The role of nuclear organization in cancer." The Journal of Pathology, 220: 114-125.
- Takei Y., et al., (2021). "Integrated spatial genomics reveals global architecture of single nuclei." Nature, 590: 344-350.
