Spatial Omics Solutions for COVID-19

The World Health Organization declared a 2019 pandemic of coronavirus disease (COVID-19), an infection caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The COVID-19 pandemic caused by SARS-CoV-2 has further highlighted the clinical need to detect infections, track strain evolution, and identify biomarkers of the disease process.

How we cover a large area of spatial-omics analysis protocols in COVID-19 studies

Spatial analysis of biomarkers of organ injury and intra-tissue immune infiltration can yield important insights when spatial omics are combined with disease progression, therapeutic response, and more traditional gene expression analysis. CD Genomics offers spatial omics solutions that enable researchers to rapidly perform high-complexity experiments in key areas of biology. To answer questions related to the impact of pathogenic infections at the molecular, cellular, tissue, and individual levels.

In addition to this, we combine the collected single-cell RNA sequencing data to generate and spatially map comprehensive cell type profiles using the cancer transcriptome atlas (CTA) and the whole transcriptome atlas (WTA) as well as additional custom content for the SARS-CoV-2 gene. This allows our clients to develop a broad molecular description of COVID-19 disease that can inform public health and potentially help develop and advance SARS-CoV-2 diagnosis, prevention, and treatment strategies.

Figure 1. Spatial transcriptomic profiles of patient autopsies. (Butler D., et al., 2021)

Our clients can obtain

• Spatial analysis of tissue samples with host immune components and the addition of custom markers for pathogens and hosts of interest.
• Allows direct visualization of viruses and estimation of viral load in any tissue and cell with a morphological background with unparalleled sensitivity and specificity.
• Can be combined with immunohistochemistry (IHC) on the same slide for targeted, simultaneous detection of RNA and protein.

Samples that can be analyzed

Our solutions can be used for a variety of sample types, including formalin-fixed, paraffin-embedded (FFPE) tissue sections, and are capable of studying human and model organism pathogenesis and host responses.

We do it better

CD Genomics offers a spatial omics technology service that can pinpoint cellular processes, expression pathways, and immune cell profiles, revealing how infected cells from multiple organs exhibit a range of molecular and genomic changes. And can identify any host or viral target genes to enhance our clients' systematic understanding of SARS-CoV-2 pathogenesis and progression and to assist in the development of vaccines and new therapies.

Reference

1. Butler D., et al., (2021). "Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactions." Nature Communications, 12, 1660.