InquiryOligodendrocytes are a subtype of glial cells responsible for the synthesis and maintenance of myelin sheaths in the central nervous system. Damage and loss of oligodendrocytes can lead to demyelinating diseases, such as multiple sclerosis. Conducting histological and regulatory pathway studies may lead to the discovery of compounds that target myelin-forming oligodendrocytes to improve our understanding of human oligodendrocyte development and facilitate the development of therapeutic strategies based on myelin regeneration.
Figure 1. Oligodendrocyte types in neocortex (CTX), grey matter (GM) and corpus callosum (CC), white matter (WM). (Markus M., et al., 2021)
How we can use spatial omics solutions in oligodendrocyte research
In situ sequencing is a targeted approach to pinpoint genes in their natural environment. CD Genomics targets oligodendrocytes using the pciSeq (probabilistic cell typing by in situ sequencing) method, which uses prior sc-RNA seq data to assign detected transcripts to segmented cells and then assigns the cells to cell types. Characterizing oligodendrocyte lineages and VLMC (vascular and leptomeningeal cells) populations in this way allows our customers to determine the spatial distribution of oligodendrocytes at an unprecedented resolution.
- Obtain the number of oligodendrocytes
- Allows detection of major oligodendrocyte populations
- Quantify the oligodendrocyte lineage in white and gray matter
- Obtains the relative distribution of oligodendrocyte types in each brain region
- Compare changes in the relative abundance of oligodendrocytes in different regions
- Obtain the cell density of each oligodendrocyte type
Sample request: brain tissue sections (pathological/physiological)
Technical advantages
- Has spatial resolution
- Suitable for in situ sequencing data
- Possibility to examine the distribution of cells in anatomical regions
- Presents spatial location, abundance, and gene expression characteristics
- Is a multiplex in situ RNA detection and identification method
- Enables neighborhood enrichment analysis
We do it better
Single-cell RNA sequencing reveals genome-wide expression patterns of many closely related neuronal types, but not their spatial arrangement. CD Genomics, therefore, offers pciSeq to identify the spatial structure of closely related cell types in the brain and other tissues, especially oligodendrocytes. Our customers can integrate our dataset with detailed histological information targeting astrocytes, microglia, and other subtypes, contributing to a better understanding of the cellular structure of the central nervous system and its established principles.
Reference
- Markus M., et al., (2021). "Spatial cell type mapping of the oligodendrocyte lineage in the mouse juvenile and adult CNS with in situ sequencing." bioRxiv, 447052.
