InquiryExposure of cells or organisms to toxic chemicals (even drugs) produces a range of regulatory responses, including changes in the levels of different types of biomolecules and regulation of interactions and feedback pathways. The use of a single histological technique (e.g., transcriptomics) can capture only a small fraction of the biological cascade of changes in a single type of biomolecule. Although single histological analysis techniques can identify certain biomarkers, they still do not provide a systematic understanding of the cell or organism in response to toxicity or adverse outcomes.
How we can use spatial histology to deepen our insights into toxicology
The molecular mechanisms of cellular spatial responses to drugs are a new and important topic in cell biology and toxicology. CD Genomics is dedicated to using spatial omics (both spatial transcriptomics and single technology approaches) to analyze the transcriptional profiles of specific cell subpopulations in toxic states, and how the organizational locations where interactions occur, intercellular neighborhoods, and cellular morphology and molecular epigenomes are functionally correlated. As well as analysis of interaction/communication mechanisms by combining scRNA-seq with the spatial information provided by spatial omics.
- Selection and isolation of target single cells by multiplexing or in situ barcoding.
- Monitor the spatial transcriptome profile of target cells at various stages of cell colonization, migration, differentiation, phenotypic migration, and response to drugs.
- Visualize the spatial track location and spatial transcriptome of cell populations, including rare cell populations, using one-dimensional scRNA-seq-based data.
- Reconstruct tissue visualization and neighboring structures by mapping cell surface receptor/ligand gene expression.
Possible technical options for implementation
We do it better
We provide scRNA-seq-based spatial omics as key support for single-cell resolution toxicology studies. It can provide positional information about functional communication and interactions between cells and reconstruct the spatial structure of the target cell-oriented microenvironment, enabling dynamic and spatial monitoring during cellular transformation.
- Multi-tissue compatibility
- Technical and performance stability
When cells interact with drugs, CD Genomics offers spatial omics services that may provide additional information on the transcriptional regulatory networks and functions of specific cell populations within tissue structures. This provides our clients with deeper insights into intercellular location and communication during changes in cellular sensitivity, toxicology, tolerance, and resistance to drugs and treatments.
