InquiryIn the limited space of the cell nucleus, the majority of the genome is tightly folded, leaving only the part that needs to be transcribed open. Changes in chromatin openness under specific conditions can provide a wealth of information on the regulation of gene expression, pointing the way to the discovery of new sites for binding various proteins, and allow researchers to analyze primary tissues and cells (e.g., islet cells), clinical patient samples, and classify patients and samples based on chromatin openness characteristics. Thus, researchers have a tremendous interest in determining which genomic regions are active in a given cell type.
We offer a single-cell chromatin accessibility solution
CD Genomics can use ATAC-seq (assay for transposase-accessible chromatin with high throughput sequencing) technology to detect open regions of cellular chromatin at the single-cell level with the help of high-throughput sequencing, providing a new solution for the study of regulatory mechanisms at the single-cell genome level.
First DNA probes (which function as transposons) are incorporated into open regions of the genome by enzymatic reactions, and then we can identify these regions by sequencing. In addition to this, CD Genomics offers a solution that includes powerful software analysis and intuitive visualization tools for the analysis of gene regulatory networks in individual cells for single-cell mapping of open regions of cell chromatin.
General process
Data analysis: we provide data analysis including sequencing data quality assessment, comparison of sequenced sequences, chromatin accessibility region identification, cell identification, cell sorting, and transcription factor differential analysis.
Sample types: cell lines, primary cells, fresh and frozen cells.
Practical applications
- Tumor typing studies
- Prediction of potential markers of disease
- Analysis of cell types and states associated with cell developmental pathways
- Understanding the mechanisms of gene regulation and cellular response to drugs or diseases
- Identification of transcription factors that drive cell fate, disease, or response related
- Epigenetic mechanism studies of embryonic development
- Functional heterogeneity study of tumors
Why choose us
- Only a few cells are required to identify all active regulatory sequences in the genome.
- The chromatin open regions of tens of thousands of cells can be analyzed simultaneously, revealing how chromatin compression and DNA-binding proteins regulate gene expression.
- High-throughput sequencing of libraries using the Illumina sequencing platform provides access to chromatin open region data from a large number of single cells at once.
- Cellular transcriptional regulatory mechanisms can be studied while addressing cellular heterogeneity.
