Single-cell Sequencing Service

Inquiry

What is Single-Cell Sequencing

Single-cell sequencing is a genomic approach to reveal transcriptomics at the individual cell level, including identifying tissue cell types as well as reflecting the cellular heterogeneity and tissue microenvironment of different samples. It allows an in-depth understanding of the state and association of each cell from bulk tissue, presenting a more realistic and comprehensive cellular analysis. Currently, single-cell sequencing technology plays an important role in the fields of oncology, developmental biology, microbiology, neuroscience, and botany and has become a focal point of life science research.

	Single-cell Sequencing	High-throughput Sequencing
Definition	Whole-cell sequencing of all cells in one cell population	Sequencing of each cell in one cell population
Detection ability	100-200 Gb (contains gene expression from 1,000-6,000 different cells)	6-10 Gb (contains gene expression of only one whole cell population)
Detection	19,000-23,000 genes (contains 1,000-5,000 genes expressed per cell)	19,000-23,000 genes expressed
Application	Include reproduction, tumor, fetal development, neurodevelopment, disease, immune research, etc.	Various routine research areas
Advantage	1. Accurately analyze the gene expression of each cell; 2. Accurate differentiation of cell populations and comparison between different cell categories; 3. Identify the expression of rare cells; 4. Analyze and interpret intercellular relationships in the microenvironment; 5. Describe samples from various perspectives, e.g., expression, cell amount, and cell composition	1. Lower cost; 2. Convenient and mature analytical process

Our Single-cell Sequencing Services

To perform accurate and rapid single-cell sequencing studies, CD Genomics has optimized our single-cell sequencing platform based on cutting-edge research literature to achieve truly accurate, reliable, and comprehensive single-cell sequencing and data analysis. We can analyze thousands or even tens of thousands of single cells, addressing the throughput or scalability limitations of conventional scRNA-seq.

Limitation of Single-cell Sequencing & Solutions

1 Difficult to obtain single cells due to various tissue requirements

Specific dissociation and suspension systems are designed for different tissues to ensure the successful acquisition of single cells

2 Frozen tissues and precious samples cannot be used for experiments

Adopt the internationally recognized frozen tissue recovery technology and MACS dead cell removing technology to ensure the sufficient use of frozen tissue

3 PCR bias interferes with gene expression in single-cell sequencing

Using advanced technology to construct PCR unbiased library

4 Single-cell sequencing analysis is difficult, and the amount of data is large.

Utilizing an advanced computing platform and performing by an experienced team of biochemical experts, we can accurately and quickly analyze single-cell sequencing data

5 Single-cell RNA classification accuracy is insufficient because certain cells cannot be subdivided

Adopted the single-cell proteome and single-cell transcriptome sequencing technologies simultaneously to achieve comprehensive detection from upstream to downstream and ensure high cell classification accuracy

Our Advantages

1. Expert in the 10X Genomics single-cell platform that is industry-recognized to reveal unbiased single-cell sequencing;

2. Extensive experience in single-cell suspension preparation for different sample types, such as peripheral blood, cell line, fresh/frozen organ tissue, and tumor tissue, to ensure cell viability meets sequencing requirements;

3. Possess mature single-cell sequencing library construction technology, complete library construction of 1000-10000 cells simultaneously, truly measure all cell types in the whole tissue, and achieve a comprehensive analysis of all cells within the sample;

4. Complete single-cell sequencing workflow, rigorous quality control procedure, and extensive single-cell sequencing and data analysis to achieve customized data analysis services.

Sample Requirements

Sample Type: tissue, blood, cultured cell, single-cell suspension

Note: CD Genomics is willing to provide technical and experimental assistance with our greatest effort for tissue samples that can be dissociated to obtain a single-cell suspension. Due to the specificity of different sample types, we will not guarantee that the experimental method can apply suitably to all populations.

Quality Requirements:

Cell viability greater than 70%

Concentration between 500-2000 cells/μL

Volume no less than 200 μL

No cell culture medium and buffer should contain Ca²⁺ and Mg²⁺

Total cell volume less than 40 μm