Spatial Omics Solutions for Inflammation

Inflammation is associated with a variety of diseases, including asthma, arthritis, cancer, obesity, heart disease, colitis, and neurological disorders, and optimal anti-inflammatory therapy is critical to the management of these diseases. Different immune cell populations and immunomodulatory pathways play critical roles in acute and chronic inflammatory responses. Detection of secreted factors and their receptor and cellular sources, as well as other biomarkers, is critical to the understanding, diagnosis, and treatment of many inflammatory diseases.

How we use spatial omics techniques in inflammation research

Spatial gene expression heterogeneity plays an important role not only in organ development but also in the pathology of various inflammatory diseases. CD Genomics offers a very reliable and robust approach to detect and validate inflammatory biomarkers in the tissue environment with in situ hybridization analysis with the help of spatial omics methods. It can be used as a powerful tool for the discovery of genomic mutations, epigenomic modifications, aberrant transcription/translation, and cellular tissue distribution. Importantly, we offer high sample compatibility with our technical services, which can be performed on routinely available FFPE samples. In addition, we also integrate novel imaging methods for high-quality visualization of inflammatory processes, giving our customers powerful tools to unlock personalized inflammation studies.

Our customers can obtain

• Identification of cytokines and their cellular origin.
• Analysis of the role of inflammatory pathways in the carcinogenesis process.
• Detection of long-stranded non-coding RNA (lncRNA) in inflammatory diseases.
• Specific detection of inflammatory markers and cytokines in the tissue environment.
• Analysis of the therapeutic potential of secreted proteins in inflammatory diseases.

Figure 1. Interdisciplinary setup of multi-omics, spatial transcriptomic, and imaging data including machine learning techniques, providing a platform for individual treatment decisions. (Anchang C. G., et al., 2021)

Technical approach

• SeqFISH+ includes a high-sensitivity single-molecule fluorescence imaging system. Its use enables our customers to detect up to 10,000 different transcripts in situ at the (sub)cellular level.
• The Slide-seqV2 dispenses dense arrays of barcode beads on a glass surface to achieve 10 μm resolution, with near single-cell level and almost 100% RNA capture efficiency.
• Transcriptome heterogeneity can be visualized at the cellular/subcellular level using the Seq-scope.

Practical applications

We do it better

CD Genomics offers services based on spatial-omics technologies that facilitate inflammation research with high technical availability, low cost, and advanced automated systems. Providing our clients with the opportunity to explore single-cell heterogeneity at an unprecedented depth and reveal patterns of association associated with the disease. And our combination of spatial-omics data analysis and improved imaging technologies provides unique advantages for the clinical diagnosis, monitoring, and treatment of inflammation, which greatly facilitates our clients' understanding of the inflammatory process.

Reference

1. Anchang C. G., et al., (2021). "The Potential of OMICs Technologies for the Treatment of Immune-Mediated Inflammatory Diseases." International Journal of Molecular Sciences, 22(14): 7506.