InquiryAssessing how pathogens infect, how hosts respond to infection, and how to develop effective vaccines and therapies remain key challenges for infectious disease research. Single-cell histology can reveal the hidden molecular systems that lead to infection and pathogenesis in patients. When combined with more traditional gene expression analysis, important insights can be generated into biomarkers of organ damage and immune infiltration within tissues. However, this is accompanied by the inevitable limitations associated with the loss of spatial location information.
How we can provide spatial-omics solutions in the field of infectious disease research
While single-cell histology can yield relevant information in the context of disease progression, traditional single-cell RNA-seq analysis is insufficient to obtain the complex information needed to gain insight into molecular behavior. CD Genomics provides solutions at different levels for the study of infection mechanisms and therapeutic interventions in different infectious diseases through technologies in spatial single-cell histology, barcode genealogy tracking, and single-cell multi-omics.
- Marker identification and spatial analysis
- Cell type classification and trajectory inference
- Quantification of viral mRNA at the single-cell level
- In situ detection and visualization of pathogens
Samples can be analyzed
No specific species restrictions (relevant to the type of disease being analyzed), and our technology platform is multisample compatible. Can be used for a variety of sample types including formalin-fixed, paraffin-embedded tissue sections, fresh frozen sample tissue sections, etc.
Technical features
- Preserves morphological context and provides average expression levels across cell populations rather than at the single-cell level.
- Can be combined with other techniques (real-time quantitative fluorescent PCR assays, IHC) to complement data information with single-cell expression levels and localization.
- For detection and visualization of viruses in the infected environment, and in the case of virus detection, preserves morphological context and can be used for virus discovery.
- With unparalleled sensitivity and specificity, and the ability to detect any gene in virtually any tissue of any species, it can be used as a routine tool for virus detection.
Our advantages
- Efficient process. Rapid workflow and rigorous and organized standardized operations allow us to be a provider of responsive solutions for the detection of emerging or exotic viruses.
- Specificity. We offer a technical strategy that enables accurate detection in highly related species/viral strains without interference from the presence of contaminants or related (similar) viruses.
- High sensitivity. Using single-molecule RNA detection and in situ hybridization, we can identify individual virus particles in infected cells, even when viral loads are low or undetectable by conventional methods. Such a high level of sensitivity can also be used for specific detection in the early stages of infection.
- Accurate localization. We provide accurate molecular detection and visualization of infected cell types and tissues, which can assist our clients in elucidating key points of virus transmission, pathogen-host interactions, and localization of hidden hosts.
- Multi-modal assays. We offer dual and multiplex assays that can detect multiple viruses or co-infections. We are also able to differentially detect and differentiate between viruses in latent or active stages.
